Does RNasin inhibit RT?

Steven Sullivan sullivan at gwis2.circ.gwu.edu
Tue Aug 29 18:19:20 EST 1995



In the course of tweaking differential display protocols, it appears that
a commonly used RNase inhibitor (Promega RNasin), added as 1 ul per 20 ul
reverse transcription reaction, seems to reduce the amount of 1st strand
product compared to identical reactions lacking Rnasin.  I've found this
to be true regardless of whether Superscript or MMLV is used as a reverse
transcriptase. Has anyone else observed this, or should I send it off
immediately to Biotechniques ? ;>





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