Western Blot

Yasushi OKADA yokada at kinesin.kaibo1.m.u-tokyo.ac.jp
Tue Aug 29 06:08:29 EST 1995


In article <41tibd$7na at net.auckland.ac.nz> a.weaver at auckland.ac.nz
(Alison Weaver) writes:

> I am having problems with the transfer of a 150KDa protein which has
> a transmembrane domain from SDS PAGE to Hybond C membrane. Does
> anyone have any suggestions how transfer may be enhanced?

Lower MeOH conc, add very low conc. (say, 0.01-0.1%) SDS.

--
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* Yasushi Okada, MD.           | Email:yokada at kinesin.kaibo1.m.u-tokyo.ac.jp *
* Dept. Anatomy & Cell Biology | Tel:81-3-3812-2111 ext.3336                 *
* Univ. Tokyo  JAPAN           | Fax:81-3-5689-4856                          *
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