help: primer restriction sites

Frank Chen yatsen at
Wed Aug 30 08:24:49 EST 1995

Dear Colleagues,
	I designed a pair of primers which have restriction sites at 
their 5' ends.  I did not add additional bp since I intended to clone 
that into a PCR vector first.  I am wondering if I can directly digest 
them with the enzymes for cloning into an expression vector.  I would 
appreciate your help if you have done that for these two enzymes.
	The enzymes are: Bam HI and Hind III.

Frank Chen

More information about the Methods mailing list