help: primer restriction sites
yatsen at wam.umd.edu
Wed Aug 30 08:24:49 EST 1995
I designed a pair of primers which have restriction sites at
their 5' ends. I did not add additional bp since I intended to clone
that into a PCR vector first. I am wondering if I can directly digest
them with the enzymes for cloning into an expression vector. I would
appreciate your help if you have done that for these two enzymes.
The enzymes are: Bam HI and Hind III.
More information about the Methods