EtoH ppt

DYDM6025 at BUREAU.UCC.IE DYDM6025 at BUREAU.UCC.IE
Wed Aug 30 04:34:11 EST 1995


Hi,
On reading an old listing 93` someone was advised not to EtoH ppt digested
DNA. The material was to be used for a S blot, the reasoning bheind this
was that the EtoH ppt had led to inconsistent results.

What other alternative can you use with a 100ul digest.
What could cause the inconsistent results, understanding there may be a loss
of DNA during the ppt.

Apologises if this has been repeated.

Any ideas,
Regards,
Sean.



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