In situ PCR

John Bussell johndb at
Wed Aug 30 22:12:51 EST 1995

Hello All
I am after some information on in situ PCR.  Is it possible to use as an
alternative to in situ hybridisation, given that my aim is to localise a
specific sequence onto a chromosome spread?  Initially I thought yes, but
I have yet to encounter any such application in the literature.  

The denaturing step of PCR possibly precludes such an application as it
appears that the PCR product is free to move about within the cell after
it is denatured from the template.

Any thoughts on this would be greatly appreciated

John Bussell                                      email: johndb at
The Department of Botany
The University of Western Australia                     phone: 61 9 3803549
Nedlands 6907                                                                     fax: 61 9 3801001
Western Australia

More information about the Methods mailing list