Cleaning glassware for DNA

Shahram Mori smori at
Tue Aug 29 20:44:36 EST 1995

Danuta Bois (dbois at wrote:
For DNA.

: 1.  How is glassware to be washed?  Is washing with detergent,
: followed by several rinses with tap and then with deionized water
: enough?


: 2.  Does glassware need to be baked?  If so, how long and at what
: temperature?

: 3.  Is autoclaving useful or necessary ?

: 4.  How does one handle anything that goes into many solutions, like
: pH meter probe, etc.

you don't need to, as long as you don't use a DNase as your probe bath :)

: 5.  Can some things, like stirring bars, be handled with gloves prior
: to use, without worry about contamination?

You don't need to wear gloves when you work with DNA

: 6.  Any other important points I may have missed.

: All comments and suggestions will be greatly appreciated.

All that you have asked is neccessary (and more) for RNA, Not DNA.
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Shahram Mori
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM

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