help on PCR from phage plugs

Kent Nastiuk, Ph.D. nastiuk at rockvax.rockefeller.edu
Thu Aug 31 14:57:26 EST 1995


  I find that including 0.05% NP-40 in 'dirty' (ie anything other than 
purified DNA as starting material) to improve the reliablility of pcr 
amplifications.

good luck




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