subtractive hybridisation

Debbie D.Carter1 at
Thu Aug 31 08:24:15 EST 1995

I'm trying to set up a subtractive hybridisation system to look at  
differential gene expression between two tissue types. I would be most 
grateful for any hints and tips. I'm particularly interested in the idea 
of using PCR to increase the amount of driver used as there is limited 
starting tissue. Any ideas will be warmly recieved.

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