help: primer restriction sites

Igor R Sagdeev isagdeev at
Thu Aug 31 05:54:07 EST 1995

The table for enzyme efficiencies near the end of DNA fragment
is great! However, the list of enzymes in the tables is very
limited. Is it safe to assume that for other enzymes (I'm interested 
in NciI and HhaI) the efficiencies are 90% or higher when the
restriction site is flanked by 3 or more bp?


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