SEQUENCING Large Plasmids

[Steve Wylie]

Has anyone tried to DNA cloned into plasmids of around 12kb? Presumably 
one would need to add more template than with a smaller plasmid as the 
proportion of target DNA to the rest of the plasmid is less. ABI tells me 
it is better not to attempt it, but I would like to check the site of 
insertion into the vector. Any suggestions most welcome.
Steve Wylie
Murdoch University
Western Australia