In situ PCR

[Gary K.]

In article <johndb-3108951108270001 at mac7.botany.uwa.edu.au>,
johndb at uniwa.uwa.edu.au (John Bussell) wrote:

> Hello All
> I am after some information on in situ PCR.  Is it possible to use as an
> alternative to in situ hybridisation, given that my aim is to localise a
> specific sequence onto a chromosome spread?  Initially I thought yes, but
> I have yet to encounter any such application in the literature.  
> 
> Possibly the denaturing step of PCR precludes such an application, as it
> appears that the PCR product is free to move about within the cell after
> it is denatured from the template.
> 
> Any thoughts on this would be greatly appreciated.



Yes there is a method for localizing specific sequences on chromosome
spreads using in situ PCR.  Check BioTechniques vol. 15, no. 1 pp. 78-80
(July 1993).

Good Luck,
Gary