pemanuel at umabnet.ab.edu
Thu Aug 31 13:09:23 EST 1995
I am having low recoveries of a gel slice containing a DNA insert. I have
a 1400 bp insert which is present at low levels. The slice may have 500
ng in it and I need all of it or as much as I can get.
Glassmilk (Geneclean) has been weird. I seem to lose a lot each time.
I have an elutrap machine I want to use now.
Can I expect that this machine will work better than glassmilk?
Will the TAE buffer crystallize when I precipiatate the 200 ul of eluted
stuff I suck out of the TRAP in the EluTRAP machine?
What can I do to get a better recovery?
Peter Emanuel Ph.D.
University of Maryland School of Medicine
4-027 Bressler Research Building
655 West Baltimore Street
Baltimore, MD 21201
More information about the Methods