Yeast chromosomal EcoRI digests

paul bundock bundock at rulsfb.leidenuniv.nl
Thu Aug 31 20:03:36 EST 1995


I'm not sure if anybody else has this problem but I seem to be unable to 
efficiently digest yeast chromosomal DNA with EcoRI.I'm using the 
recommended buffer and a large digestion volume of 200ul.The DNA is 
purified using a lyticase protocol with many phenol steps.Does anyone 
have experience with this? Thanks, Paul Bundock.




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