Setting up a lab at home
ALEXANDERW at cber.cber.fda.gov
Thu Aug 31 04:46:28 EST 1995
In Article <423qvi$2401 at news.cuny.edu>, kang at msvax.mssm.edu wrote:
>In article <41vlar$maq at hermes.oc.com>, Gordon Betts <betts at orion.etsu.edu>
>>Susan Jane Hogarth <sjhogart> wrote:
>>> I'm considering setting up a basic "molecular biology" laboratory in my
>>> hope to design/build much of the equipment, and to purchase some. This is
>>> as far-fetched as it may seem (I hope!) as my mate is a
>>stuff deleted --
>>> Susan Jane Hogarth
>>> sjhogart at unity.ncsu.edu
>>About a month ago there was a discussion on this group about $1
>>electrophoresis. Check the archives.
>That was not $1 electrophoresis. That was less than $1 gel box.
>I do not want to set up whole lab in my home. Rather, I want to have small
>facilities simply because I do not want to stay at lab late to just transform
>If I want to subclone something, this is my typical SKD.
>10:00 AM; Think some good strategy
>10:30 ; Start RE digestion
>12:30 ; Running gel (having lunch during this time)
>01:30 PM; Start DNA purification from the gel
>02:00 ; Start ligation
>03:00 ; Mix ligation mix with competent BUGs, keep cold
>03:30 ; Heat shock and add medium, keep warm
>05:30 ; Plating
>Oh! If only I have a nice and little incubator which can give sufficient warmth
>to my little fellows, I don't have to stay at the lab till 5:30. I can bring my
>little fellows in my pocket and can plate them in my home lab.
>Inventor of the less than $1 gel box.
Try pour plating your transformants directly after heat shock using top-agar
without antibiotics. This will cut out the recovery time, therefore plating
should be finished at 3:45. Now you can start another experiment! ;-)
More information about the Methods