SEQUENCING Large Plasmids
jgraham at bronze.ucs.indiana.edu
Thu Aug 31 17:05:33 EST 1995
When faced with this problem, I decide to add an F1 origin and generate
11 nt single-stranded circular plasmids for sequencing. Pharmacia's
PucF1 vector provides a phage origin within a large polylinker.
Even large single stranded circles required gel purification
after heat-SDS liberation of the DNA for clear sequencing. Contact
me directly if you need to do this.
J. Graham PhD
Washington University of St. Louis
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