fluorography?

[NICHOLAS THEODORAKIS]

In article <425iaa$gha at azure.acsu.buffalo.edu>,
Xin Guo <xinguo at acsu.buffalo.edu> wrote:
>Hi, a method I need to know for my journal club (student). The method
>in the paper said 35S-labelled GST fusion proteins were eluted from
>Sepharose beads, separated by SDS-PAGE, and visualized by
>
>FLUOROGRAPHY.
>
>So, what is fluorography? I know it isn't autoradiography or ECL. No
>any details and references in the paper. The pictures in the paper
>look like regular western blot pics with whatever detecting methods.
>
>Thanks for helps.
>
>Xin 
>SUNY at Buffalo
>-- 
>------
>long axons and short dendrites      ~{VaM;3$!!JwM;6L~}
>http://www.acsu.buffalo.edu/~xinguo

Man, am I starting to feel old.  What are they teaching kids these days?
Anyway, fluorography is a method for enhancing detection of radioactivity 
with a scintillating fluor that is impregnated into the gel.  The film 
then detects light that is emitted when the fluor is excited by beta 
particles.

see Bonner and Laskey (1974) Eur. J. Biochem. 46:83-88.

Nick "who still knows what PPO is and how to use it" Theodorakis


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			Nick Theodorakis
			ntheo at welchlink.welch.jhu.edu
			Johns Hopkins Medical School, Baltimore, MD