M13: plates are all blue!

Jo Caine JO_C at vcp.monash.edu.au
Thu Aug 31 17:56:45 EST 1995


Hi,

I've just done a M13 transformation and plated out in top agar the lawn cells, 
IPTG, X-gal and the transfored cells.  This morning all the plates are a 
lovely shade of blue.  The lawn is intact and there are some plaques, but I 
can't tell the color of them because of the blue background.  
Has anyone got any explaination?  The same transformation worked last week.  
Could one of the reagants gone off in that time?

Thanks in advance.

Jo Caine
Victorian College of Pharmacy,
Monash University.
Melbourne 
Australia





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