Western Blot

[Jim Hutchins]

Pamela Norton (pnorton at lac.jci.tju.edu) wrote:
:     My curiosity has been sparked by this thread. I don't have extensive
: experience with blotting many different types of proteins, but we routinely
: transfer proteins of >200 kDa in 20% MeOH, no SDS. How much is transfer
: affected by the hydrophobicity of the protein in question? (ours are not
: very). Is there a good reference for this sort of thing?

Pam,

Glad you asked :-)

Van Oss CJ, Good RJ and Chaudhury MK.  Mechanism of DNA (Southern) and protein
(Western) blotting on cellulose nitrate and other membranes.  J Chromatog
391(1):53-65, 1987.

I highly recommend this paper.  The biophysics is a little slow going, but
you can get the sense of it from the summary paragraphs at the end of each
section.

--
Jim Hutchins
Assoc Prof Anatomy, Asst Prof Neurology (Research), Univ Mississippi Med Ctr
http://fiona.umsmed.edu/~hutchins/    ***    E-mail:   hutchins at umsmed.edu
``It became necessary to destroy the town in order to save it.''--American
infantry officer firing on Ben Tre, Vietnam, 2/8/68