Ca Phosphate Transfection/HUVEC Cells

Christopher Wong Wongc at a1.mscf.upenn.edu
Thu Aug 31 17:25:31 EST 1995


HELP HELP HELP

Have been trying to transfect plasmids into HUVEC (Human Umbilical Vein 
Endothelial Cells) with minimal success.  Would appreciate any help/
suggestions regarding:

1) Protocols for transfection of HUVEC by calcium phosphate or other 
methods

2) Optimization of calcium phosphate transfection for difficult to 
transfect cell lines

Thank you in advance

Jim Kallman
kallman_j at a1.mscf.upenn.edu> 






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