Antibody for His-Tag
lally at toxin.dental.upenn.edu
Thu Aug 31 17:05:23 EST 1995
In article <tomasd-0609561722140001 at fp5-9n.domain>, tomasd at bdg10.niddk.nih.gov (Tomas Drgon) writes:
|> They say it "sees" Met-Arg-Gly-Ser-(Hisx6). So it might see also (Hisx6) alone.
|> Did you try it out?
Didn't need to do it, the Ni+-NTA-PO4 worked fine for me.
Six tandemly expressed His are no doubt very immunogenic and since an antibody
combining site is 6-8 aa there is no doubt that some of the antibodies recognize
the His-tail. But how much, I don't have any idea, I didn't make the antibody.
Qiagen recommends the antibody on vectors expressing Met-Arg-Gly-Ser-(Hisx6) you can bet
that that a significant part of that titer is tied up in some part of Met-Arg-Gly-Ser.
What do you think??
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