Does RNasin inhibit RT?

mhughes at mhughes at
Thu Aug 31 19:34:57 EST 1995

In article <42079o$kus at>, sullivan at (Steven Sullivan) writes:
> In the course of tweaking differential display protocols, it appears that
> a commonly used RNase inhibitor (Promega RNasin), added as 1 ul per 20 ul
> reverse transcription reaction, seems to reduce the amount of 1st strand
> product compared to identical reactions lacking Rnasin.  I've found this
> to be true regardless of whether Superscript or MMLV is used as a reverse
> transcriptase. Has anyone else observed this, or should I send it off
> immediately to Biotechniques ? ;>

	We have been using RNasin (Promega) in out RT reactions here for quite
awhile.  We use AMV RT from Life Technologies and don't have a problem getting a
good deal of cDNA, leaving it out though, does seriously affect our product. 
That might reflect the RNA source more than the reaction. Who would know.


More information about the Methods mailing list