Methods August 1995 Archives by thread
Starting: Tue Aug 1 01:02:10 EST 1995
Ending: Thu Aug 31 22:29:13 EST 1995
Messages: 1329
- seemingly two bands of one band in SDS-PAGE?
Soon Paik
- northerns
Soon Paik
- Clontech TCR typing amplimer kit
Kate Platts
- Test
Michael Strehle
- pTTQ18
Duncan Clark
- In situ on single bacteria?
sbutcher at molbiol.ox.ac.uk
- Mutagenising phosphorylation sites
David Micklem
- Rb.sphaeroides genomic library
M.D.Omrani
- strataclean--homemade version?
Scott Vande Pol
- Recommendations for software to support small sequencing project
Eric C. Anderson
- CAT assays and phosphoimagers...
batu erman
- Searching for BACHEM
Holger Bohnemeier
- RT-PCR and gels
Holger Bohnemeier
- infor. on PCR machine
Anton Scott Goustin
- Low copy # reporter?
Richard Vernell
- fungi in the incubator
Betty-anne Mcbey
- HELP: inconsistent genomic PCR troubleshooting
QIAGEN
- pET vector cloning of hydrophobic protein gene
Mike Holloway
- RT-PCR and gels
Gordon Betts
- Endonuclease activity: Crude extraction from E.coli
Julian Wood
- CAT assays and phosphoimagers...
Traci Roth
- DNA isolation from bone
Claus B. Jorgensen
- Mettler microbalance for sale
Susan Boyle-Vavra
- (none)
Jeff Kramer
- Low copy # reporter?
Brad Nicholson
- Recommendations for software to support small sequencing project
Michael Myers
- omission mutagenesis
Elias Fernandez
- Ebola virus speakers needed
R. Dana - Green Gene
- Trouble with a Millipore "Expedite" Oligonucleotide synthesiser
Greg Reynard
- (no subject)
Peter Hains
- Fluorescent phospholipid
Peter Hains
- Email Address of Boehringer
h.woo
- mtDNA specific PCR primers
Henk Pennings
- ATCC book on freeze drying methods
DPrince225
- need some help starting molecular biology
Hans-Georg Klieber
- PCR of lambda gt10
mcelverja at phibred.com
- SuperSignal CL-HRP for Nothern & Southern
U12201 at uicvm.uic.edu
- Supressible URA3 marker
Karthi Rama
- pSPT64T
Alan Hair
- pET vectors, good or bad?
Karen Browning
- DIG Gelshifts???
Eric Hanson
- Address of HT Biotechnology?
ik103 at hermes.cam.ac.uk
- Gradients with automated DNA sequencers?
HARDIES at THORIN.UTHSCSA.EDU
- microtiterplates for sequencing
U12201 at uicvm.uic.edu
- RT-PCR and gels
Jim Hutchins
- Microfabrication Technology meeting announcement
Cambridge Healthtech Institute
- apology/expalanation re: TIBS post
Eric C. Anderson
- need a bidirectional promotor
Glenn Randall
- infor. on PCR machine
bmay
- Genomic DNA from Campylobacter
Jayant S. Aphale
- Gradients with automated DNA sequencers?
tom chappell
- NMU and tobacco
ynakao at kean.ucs.mun.ca
- More on Boomerang DNA Amplification
Paul N Hengen
- quantity of cDNA in PCR
RMCMAH93 at IRLEARN.UCD.IE
- Protein Truncation Assay
John Maris, MD
- pET vectors, good or bad?
Jeff Kramer
- Gradients with automated DNA sequencers?
Tony Murray
- Gradients with automated DNA sequencers?
HARDIES at THORIN.UTHSCSA.EDU
- infor. on PCR machine
mcelverja at phibred.com
- I have a student interested........
Gordon Betts
- RFLP of birds
Gordon Betts
- Library screening alternatives?
Steven Sullivan
- FLAG epitope - sources and advice
David Clayton
- Is there a shareware plasmid drawing program?
Laurent Seroude
- DNA methylation - effect on transfectoma expression
Immune Response
- Is there a shareware plasmid drawing program?
Lou Alarcon
- /\\/\\ Oligo melting point calculation question /\\/\\
Paul St. Amand
- amp resistance gene
Paul N Hengen
- test, please ignore
sievert at molbio.mu-luebeck.de
- Video Photodocumentation of gels
Pierre_Talbot at Infopuq.UQUEBEC.CA
- infor. on PCR machine
Jan Oliver Koch
- test, please ignore
Volker Sievert
- Plasmid Sequence Database?
Alex Morla
- Is there a shareware plasmid drawing program?
Shin-ichiro Hiraga
- HELP: inconsistent genomic PCR troubleshooting
Giorgio Spagnol
- Consultant company stalks this board
Giorgio Spagnol
- Consultant company stalks this board
Giorgio Spagnol
- Oligonucleotides
MARK PEGGIE
- PCR of lambda gt10
Giorgio Spagnol
- What is SELEX?
Stephan Witte
- RNA denaturant: methylmercuric hydroxide
Niccy.Scholfield
- DNA purification from agarose gels
f.r.byrne
- looking fo E.coli RY13
Ali Karami
- strataclean-homemade version
lidaniel
- What is SELEX?
Mikhail Alexeyev
- pET15b ligation probs.
James Smith
- lambda gtWES
MIcha Ron
- Rapid DNA Ligation Kit ???
klaus.lun at student.uni-tuebingen.de
- Summary of strataclean prep protocol
lidaniel
- pET vector problems
John Brennand, Tel 4639/5739/6636
- Ecoli Strain for protein expression
huange01 at mcrcr6.med.nyu.edu
- RNA Extraction (RVCs)
David Glover
- Non-radioactive Southerns and northerns-help
S. L. Rennie
- need help: QUICK & DIRTY RT-PCR
SEHuang
- need bidirectional promotor
Glenn Randall
- Cloning "Long Range" PCR products
Gamal E. Osman, Ph.D.
- Induction by IPTG
Andy Kravetz
- Mg++ Beads
Greg Schaefer
- paired domains?
Billy B. Long
- DH5alpha cells and MDCK cells
GORDON BETTS
- Help!!!! how to clone a PCR fragment?
Heidi Moss
- QUICK & DIRTY RT-PCR
Andrei Popov
- Non-radioactive Southerns and Northerns
Helen McBride
- UV Sterilization
Celinn
- postdoc position-wanted
stein at DOLPHIN.UPENN.EDU
- autophosphorylation question
BARTHOLOMEW PETER J
- Help!!!! how to clone a PCR fragment?
Dr. Colin Rasmussen
- DIG gel shift. Good or bad?
Brendan McMorran
- LongRanger on Automated Sequencer?
hollingswor at immunex.wa.com
- Spirulina platensis strain
gliang at miavx1.acs.muohio.edu
- Needed: Sigma Gel clone app. for Mac
Efrain Gonzalez
- peptide synthesis
Tom Anderson
- (no subject)
rey garcia
- buffer for DNase treatment?
rey garcia
- pAX vector
Dr I.M. Clark
- PC Gene
Dr I.M. Clark
- plasmid nomenclature
Kerstin Waigand
- sequence of pT7-5
Ursula Schell
- I 125 label for antibody
T. Drake
- ECL-kit Reagents
j.r.sayers
- Genetrapper Gibco?
j.r.sayers
- PC Gene
Bart.Nelissen
- Cloning Vector Help !
Richard C. Ogden
- RNA denaturant: methylmercuric hydroxide
Parke K. Flick
- Help recovering 200bp DNA fragment
Harshinee Wijesinghe
- help for direct sequencing!!
Lygia da Veiga Pereira
- RNase protection using PCR products
HOFSTRA ANGELA 905-822-3520
- Protein truncation assay.
JOHN MARIS
- help needed with southern
PAUL CANNNISTRARO
- Charon 4a flanking sequence
Michael Gorry
- help needed with southern
martin LEACH
- No way.
To: Nikolai
- (no subject)
Anna Lau
- Transcription Factor info needed
martin LEACH
- acrylamide gels and DNA markers
Dr. Kimberly Walker
- heating/cooling block???
CLEPPER at MAINE.MAINE.EDU
- pKIL18 or pKIL19: Does anybody have them?
Viraj Master
- Baculovirus Question
Huseyin Kucuktas
- liposome
edwin alcantara
- SIGMA address
Sigma BioSciences Technical Service
- Help recovering 200 bp DNA fragment
Sergey V. Orlov
- pseudovitamin b12
Jeff Lawrence
- Help - RNA isolation
Volker Sievert
- help needed with southern
Sergey V. Orlov
- Automated online journal searching
Duncan Clark
- Help in SDS-PAGE
Giorgio Spagnol
- microtiterplates for sequencing
Mr SMNN Faruque
- Background in immunostaining. Help.
Giorgio Spagnol
- A source of email/www address HERE
martin LEACH
- Lac-Z Staining Sensitivity?
veronica narvaez
- Background in immunostaining. Help.
To: Nikolai
- microtiterplates for sequencing
To: Nikolai
- HELP: RNA isolation from cell cultures
SEGALL,LAURA,MS
- Info on restriction enzymes
BRADLEY GOTTFRIED
- Manuals on CDROM
Rob Jordan
- Has anyone used the non-radioactive protein kinase c assay?
Raymond Chang
- Wanted: Plasmid encoding Luciferase
Stephan Witte
- pSRalpha
anne-claude
- RNA gels - staining and denaturing
Oleh Kovalchuke
- ABI 377 Sequencing Reagents HELP
Rick Thorne
- Wanted: Plasmid encoding Luciferase
Colin Rasmussen
- Help recovering 200bp DNA fragment
(Dave Johnston)
- help! non-radioactive Southern
Zhengyang Wu
- Help in SDS-PAGE
f.r.byrne
- Transfection of B cell-lines
r.p.yeo at reading.ac.uk
- Help! Polyclonals to a polypeptide
Steve Dunn
- B cell transfections
Melanie Brazil
- Transfection of B cell-lines
Laurentiu COCEA
- B cell transfections
Seth Corey
- How to detect GFP via FACS?
Laurentiu COCEA
- Wanted: Info on Spiral dilution plater
crawford at bscr.uga.edu
- cDNA synthesis.
Peter Nilsson
- DNA from paraffin-embedded tissue
Peter Nilsson
- Control PCR primers
Rod Minchin
- DNA CHIP
Pierre BREESE
- SDS-PAGE help badly needed
f.r.byrne
- tritium labelling of peptides
hs0lbe
- Ethanol/Coumassie?
David R. Rockhold
- ApoTag Kit
Russ Boggs
- help! non-radioactive Southern
Guilherme Oliveira
- Rat cDNA libraries - large inserts required
Dave West
- Ethanol/Coumassie
Andrei Popov
- Sequenase/PRISM dye terminators on ABI 377
Bengt Oxelman
- French Press Part Needed ASAP!!!
The doctor is IN.
- ApoTag Kit
David Voehringer
- DNA CHIP
Brett Levay-Young, Ph.D.
- Using [3H]Tdr labelled DNA on Agarose Gels
syema at welchlink
- literature/algorithm DGGE melting curves
Niels Groot
- Need colorimetric assay to detect PCR products.
Ian J. Mehr
- Non-radioactive Riboprobe-Help
sabah hussain
- French Press Part Needed ASAP!!!
jmp at jpatti.ibt.tamu.edu
- French Press Part Needed ASAP!!!
jmp at jpatti.ibt.tamu.edu
- French Press Part Needed ASAP!!!
jmp at jpatti.ibt.tamu.edu
- French Press Part Needed ASAP!!!
jmp at jpatti.ibt.tamu.edu
- French Press Part Needed ASAP!!!
jmp at jpatti.ibt.tamu.edu
- French Press Part Needed ASAP!!!
jmp at jpatti.ibt.tamu.edu
- French Press Part Needed ASAP!!!
jmp at jpatti.ibt.tamu.edu
- PhiX174 Amplication
Andrew Reum
- Help! Panning
tokio nakane
- pGem4 Maxi-prep problems
Simon Hansom
- Human erythroleukemia cDNA library
Dave West
- troubles with pUC18
David D Morgan
- Purification of egg yolk antibodies
Frank Neumann
- Re cDNA synthesis
John Brennand, Tel 4639/5739/6636
- More pET problems
JG. Head
- Arabidopsis library in pACT...
Barry Causier
- Arabidopsis library in pACT - Part Deux
Barry Causier
- Purification of egg yolk antibodies
Michael Bausher
- *** TRizol LS for Tissue RNA ***
B.Schonhoft
- test
s.k.sreedharan
- Transcription Factor info needed
rmoldwin at midway.uchicago.edu
- Help! Panning
Brett
- San Saclay??
awb at aber.ac.uk
- direct PCR from bacterial colonies.
bch127
- Computational Molecular Biology Workshop
Iosif Vaisman
- *** TRizol LS for Tissue RNA ***
HOFSTRA ANGELA 905-822-3520
- no way
JMARTINEZ at AZCC.ARIZONA.EDU
- Annealing oligos for adapter preparation
dilip
- RNA gel help
STEVE MADISON
- Using 35S in RNase protecti
Dov Shiffman
- Home-made GeneTrapper
Pin\
- PCR Artifact?
Gordon Betts
- pGem4 Maxi-prep problems
Brett
- Help with homemade lambda marker please........
Bruce McDonald Lab
- Urgent need for cloning vector with particular order of sites
Peter Russell
- heating/cooling block???
Hemant Chickarmane
- PCR Artifact?
Steven Sullivan
- lafmid BA plasmid
marcvdc at lmb1.rug.ac.be
- help! pcr of mtDNA control region
Julia54601
- HIV reagents wanted
Seth Pincus
- Baculovirus expression heterogeneity
BIOTEC54
- HELP WITH NON-ISOTOPIC DETECTION!
converrl at ucbeh.san.uc.edu
- HIV reagents wanted
Klaus Lehnert
- HIV reagents wanted
Klaus Lehnert
- Ni-affinity purification and reducing agents
Per Mygind
- DNA chips
Pierre BREESE
- DNA chips
o. van-ham
- Help:PCR GC rich region
Elizabeth Bates
- Help:PCR GC rich region
Elizabeth Bates
- Dextran Sulfate 10% vs 5%
J. Patrick Martinez
- Ni-NTA and EDTA?
Frank Neumann
- PROMEGA PinPoint System
Gerald Walter
- DNA Strider
B.Schonhoft
- anti CAT antibodies needed
Nick Vercammen
- Thermophilic DNA library?
Steve Bryson
- DNA Strider
Brett
- Frontiers in Bioscience, an electronic journal and virtual library
SIAMAK TABIBZADEH
- DNA Strider
To: Nikolai
- pEF-BOS high expression vector
AEKELLY at Gems.VCU.EDU
- Ni-NTA and EDTA?
To: Nikolai
- autorad mystery
James J. Campanella
- GenHunter Corp.: Phone/Address
Jim Hutchins
- Subscribe
Yue Hao
- HELP!! Looking for a E. coli strain
debbie tien
- RNase protection probe activities
Colin MacD
- Immobilization of Enzymes
Lawrence Normie
- searching for keratinocyte cDNA library
Pamela A. Rose
- Ab against Cd18
Giorgio Spagnol
- End cloning
oRutebuka
- Inhibitor removal (humic/fulvic acids) in ancient DNA-PCR amplifications
Vicky Chang
- disposable Scissors?
Raj Shankarappa
- Which Long PCR kit?
kamin johnson
- HIV reagents wanted
Anton Scott Goustin
- HIV reagents wanted
Anton Scott Goustin
- RBC prep
Jfloring at aol.com
- looking for seed
lees at SASK.USASK.CA
- Advice wanted on DIG gel shift kit
Brendan McMorran
- Ni-affinity purification and reducing agents
Stephan Witte
- how about bionet.molbio.pcr?
f.r.byrne
- pH growth optimum of bacteria
DR A HARRINGTON, BIOCHEMIE, TEL X482 TYGB
- amplification of 13Kb DNA fragment by PCR
San-pin Wu
- Frontiers in Bioscience, a journal and virtual library
SIAMAK TABIBZADEH
- Codon usage tables
mjb1 at Lehigh.EDU
- Wanted: kan or tet resistance cassettes for plasmids
Bernard Murray
- Home made geneclean??
Vitor Warwar
- pCMV5
Chem Lab
- pCMV5
seppen at amc.uva.nl
- pH growth optimum of bacteria
"Marianne Leverone ", BIO
- MELTING CURVES, DGGE, SOFTWARE OH MY!
Erythrochaetophile
- Synthetic oligonucleotides
Ron Caspi
- Codon usage tables
vcore
- VM-26
Dave Gilbert
- Codon usage tables
Herve Recipon
- PLEASE HELP = mRNA EXTRACTION
Gabre' Kemp
- dNTP mixes and smears
Robert M Bellin
- Losing pieces of plasmid
Brett
- pcr from lambda-gt11
Shoumo Bhattacharya
- Looking for info on DataGenetics
Jim Golden
- Non-radioactive PCR
rvdeshp0 at INTNET.UPJ.COM
- Need GTPase for positive control
John A. Newitt
- Questions about F'plasmid in male E. coli strain.
Qiaoping Yuan
- SSCP on ABI 373
Michael Gorry
- Schleicher & Schuell,Inc phone number??????
MICROPLATE
- UFmembrane testing
MICROPLATE
- amplification of 13Kb DNA fragment by PCR (fwd)
San-pin Wu
- Glioblastoma and Antibiotics
Sean Stevens
- Info about genetically modified food
Harms
- G+C content in Eucaryote
lees at SASK.USASK.CA
- Has anyone used Clontech rat IL-6 primers?
Mary P. Remington
- Sequence analysis programs.
JOHN MARIS
- separating 2,8 kb from 3,0 kb
Andreas Enger
- LG 90 bacterial strain
mazella
- ABI PRISM 310 Genetic Analyzer
Peter Kuhnert
- H-2Kd clones
Mr. C.J. Lord
- PCR from colonies
Volker Blaschke
- Plasmid software for windows
Hendrik Ballerstedt
- out-of-stock items
Denni Schnapp
- INTRODUCING BIOSUPPLYNET-An Interactive Information Source
Don Corn
- How to quantitate DMSO left in cell culture?
Helen McBride
- immunocytochemistry in monocots vs dicots
Nancy A. Markley
- ?He: gelatin block for Westerns?
Ted Davis
- need help getting started with peptide antibodies
Jonathan A. Reiss
- Phospholipase C Substrates
mark mcclain
- Help!! E coli fusion protein expression!
huange01 at mcrcr6.med.nyu.edu
- Q: International Fellowships
David Haydon
- Direct PCR of Bacterial Colonies
schoenfeld at MSSCC.MED.UTAH.EDU
- (no subject)
ramalingam t.s
- C5-propyne pyrimidines
Immune Response
- Need email of Invitrogen
Richard Foster
- dye for DNA
lasgo.bbs at AIDEBBS.EDU.TW
- Newsgroup: Bionet.jobs?
Afzal Siddiqui
- COLOMETRIC ANALYSIS
The Big Guy
- multiple runs of PCR?
Fang-Yao Hou
- Chloroquine gels
Jess Vasina
- Manual needed
Giorgio Spagnol
- Kodalith Film Problem
David J. Gerrick
- Sma I religation problem
- Sma I religation problem
Martin Osterhoff
- HELP -DNA extraction from phage
Martin Osterhoff
- Transfection of cell suspensions
newaac
- pUCSV3CAT
Alan Cheng
- Anyone using Promega MMLV-RT?
Niccy.Scholfield
- Fusion protein expression in E.coli. C.Baker
Anonymous
- RNA from fat tissue
Torsten Boerchers
- PCRs and gene probe assays in filterplates
gbarney at ozemail.com.au
- RE RNA from fat
John Brennand, Tel 4639/5739/6636
- PCR-SSCP and 35S
ynakao at kean.ucs.mun.ca
- Who sells methylmercuric hydroxide? (CH3HgOH)
Jay G. Calvert
- Alf vs ABI Prism 310
varyc.mmcri at mmc.org
- Calling all Rats!
Jackie Dudley
- Calling all Rats!
Jackie Dudley
- Why use LMP agarose gels?
James Smith
- OPC column purification of oligos
Michael Gorry
- Idaho Technology-Capillary PCR
Edward Theodore Michelini
- METHYLTRANSFERASE genes/activities?
Mai xu
- DNA Footprinting ( using DNAase)
damania
- isolation of DNA from rat blood
doering_t at mpimg-berlin-dahlem.mpg.de
- C-5 propyne oligonucleotides
Rob Steele
- DNA from human bladder
doering_t at mpimg-berlin-dahlem.mpg.de
- HELP NEEDED- 35S in RPA
Dov Shiffman
- Who sells methylmercuric hydroxide? (CH3HgOH)
Bradley Turner
- SDS-PAGE problems
Edward Theodore Michelini
- Hoefer Address needed
Giorgio Spagnol
- PCR directly from bacterial colonies
Hecht
- Help:Protocol for intracell. Ca++ meas. in myocytes and PMN-cells
Michael Strohdeicher
- amplification of 13Kb DNA fragment by PCR
San-pin Wu
- Adenovirus-- Optimum conditions for ProteinaseK req.
Unknown
- RNA from T-cells
Volker Blaschke
- Questions for glycobiologists
Iain Wilson
- New ABI sequencer?
Andy Phillips
- Expression library screening with fusion proteins
Petri T. Kursula
- TdT vs. Kinase
Steven Goldberg
- Why use LMP agarose gels?
Brett
- Xcm-1
Don Haut
- Company for transgenics
Koren Mann
- Filters for UV stains
Bernard Murray
- Hoefer Address needed
Frederick Garbrecht
- Help with PLP Mutagenesis
Steven_Burghart at BAYLOR.EDU
- RNA from T-cells
Guilherme Oliveira
- archive location?
Jeffrey N Masters
- How to find an SH3 domain
Jeffrey N Masters
- patent search
Torsten Boerchers
- Company for transgenics
Bradley Turner
- Expression library screening with fusion proteins
andre
- How to search transcription factors database?
Sara Contente
- Help: RT-PCR primer design
David Yu
- Inducible euk. expression vectors
John Moffat
- Polyethylene glycol - quantification?
Mays, Peter
- Phylogenetic Tree Analysis
bell06
- OLIGO SYNTHESIZER
DSF
- Complexing of hydrophobic proteins
l14y at unb.ca
- H.Pylori complete genomic map
Bradley Turner
- Source of DrdII??
Jeff Cohlberg
- Need info about PRIMEGEN software.
Nathan Lange
- Strains of phage T4
Rafael Maldonado
- Please recommend the journal to submit.
KIm doo yeon
- HELP: finding e-mail address
yskim at CAMD2.KKPCR.RE.KR
- native PAA-Gel on A.L.F.
Cornelia Grosse
- in situ hybridization
Uwe Harmening
- Direct cell to cell Yeast-E.coli plasmid shuttle by electroporation?
John R. Thompson
- Availability of Exo.VIII (recE) and/or RecJ
Juergen Sasse (Path)
- Help Primer Extension
Mr. J. Membrillo-Hernandez
- Help in PCR sequencing
Mr. J. Membrillo-Hernandez
- Direct cell to cell Yeast-E.coli plasmid shuttle by electroporation?
Adam Greene
- Cary 210 Spectrophotometer
Joe Sandy Faulkner, Jr.
- [Q] Products for PCR solid phase strand separation?
Stephane Vuilleumier
- DNA concentration
brofft at bscr.uga.edu
- CIP or CAP ?
Evp.
- Jouan gradient makers?
David J. Meyer
- Hypothalamic cell lines?
Steve Mcclue
- Stratagene's XL2-Blue ultracompetent cells?
AL R. PLUMMER
- DNA dyes
Brian_D Lanoil
- k.lactis library
edited
- inside-out mito-vesicles
fester at mpimg-berlin-dahlem.mpg.de
- DNA extraction from formalin fixed tissue?
Veronica Burk
- Frequently Asked Question (FAQ) list
Paul N Hengen
- Source for cDNA probes????
jim at LUCY.TLI.ORG
- archive location
Jeffrey N. Masters
- Help in obtaining Journal Article
martin LEACH
- high molecular inhibitor of CYP1A1
Ralf Neunaber
- apology
- k.lactis library
- Tm's of oligo dT primers
Steven Sullivan
- cell fusion
Danny Altshuler
- need help with cloning by pcr
Lise Bonin
- DNA dyes
Jfloring at aol.com
- need help with cloning by PCR
Lise Bonin
- help with pcr cloning
Lise Bonin
- lipid fractioning
Zhu Congju
- HELP: RNA isolation from cell cultures
Suzuki Kazuhiko
- Screening a phage display cDNA library using antibodies?
Zhonglin Chai
- PCR cloning in pDK101 XcmI, reference
Russell L. Malmberg
- address request
Stefano casalotti - ST
- adress of innovative research of america
Stefano casalotti - ST
- Tm of oligopurine/oligopyrimidine hybrids
Olav Hungnes
- Omission mutagenesis
RVDESHP$ at PWINET.UPJ.COM
- marathon kit experience?
Susanna Malmstrom
- Tunicamycin and COS 7 cells
Nigel Kenward
- Millipor/Supelco gel elutions
bio_hannan at emuvax.emich.edu
- (no subject)
Patrick Matthias
- Source for cDNA probes????
Richard A. Lawrence
- DNA dyes
HENRY DAUM
- EtBr staining of RNA
D L Clarke
- XPOUND Program??
Yun-Long Du, Ph.D.
- radioactive contamination in YOUR lab!!
Jeffrey Mahr
- Zymolayse Source
Richard Friedman
- DNA concentration
Giorgio Spagnol
- subtraction library kit?
mbracey
- Zymolayse Source
Nancy Alexander
- HELP: Is there a kit for MAxam-Gilbert Sequencing?
damania
- ?? ROBOCYCLER ??
Wsargent
- dna extraction
Benke/Belmar Login
- Please help me about BUFFER
Nanthanit Chosungnoe - SCMI - 3536468
- BRL RNA ladder hybridizes to my probe
Lana C Stallard
- Zymolyase Source
NAME RAVI PRAKASH .D
- Freezing damage DNA?
the End
- Transcription factor info foundd
martin LEACH
- Help on HPLC
Zhifeng Qin
- REQUEST: SP6 promoter in M13 phage vector
Alberto I Roca
- Homemade Qiaex?
Linton
- Formula for Borate Citrate Phosphate Buffer
Nanthanit Chosungnoe - SCMI - 3536468
- Subscription
Wen-shyong Tzou
- Cm-R or Gm-R cassette?
t.barnett at path.utas.edu.au
- I want to join the bionet.
K.Kuroda
- E1A- and bcl2-gene wanted
P. Friedl
- Home-Made Jean-Kleen
Nigel Kenward
- photographing GFP
futers at biovax.leeds.ac.uk
- Method for RT-PCR?
Mark Griffiths
- direct cell to cell yeast-E.coli plasmid shuttle by electroporation
Mikaela C Gronholm
- RNA Differential Display
Robyn A. Kerr
- PPAR gamma
M Kockx
- Robocycler
Paul Linehan
- Dots in sequencinggels
Dept of Physiological Botany
- transient transfection and panning
Seth Corey
- Sequencing Primer Extension Products
S.A. Frantz
- Sequencing problems
Andrew Cannons
- ------chromosomes for f.i.s.h.-------
gedmunds at kean.ucs.mun.ca
- mRNA in situ hybridization
jim at parasit.lan.mcgill.ca
- mRNA in situ hybridization
jim at parasit.lan.mcgill.ca
- GFP improved: e-mail needed
otello at sc2a.unige.ch
- A new Bovine papillomavirus Vector
Changcun Jia
- Restriction digestion of PCR fragments
B VISSER * x2818 Plantkunde *
- Linkers from cDNAs?
the End
- Colony screening with PCR
BOIS, DANUTA H
- Strand-specific 32-P probe by PCR????
Karl Fischer
- Sequencing problems
steven at MSHRI.ON.CA
- Restriction digestion of PCR fragments
Mike Dalrymple
- G50 remove hot oligos?
the End
- nested deletions
R. Woodward
- Expression of antibody in yet
Dooyeon Kim
- Restriction digestion of PCR fragments
Andy Kravetz
- Restriction digestion of PCR fragments
Sailesh Surapureddi
- Government Restricts Internet!!
Tony Murray
- Sequencing problems
Technical R&D
- Strand-specific 32-P probe by PCR????
neale at mbcf.stjude.org
- Is M13K07 a high copy number in DH5a?
Qiaoping Yuan
- Good Marker for Histiocyte and/or Macrophage ?
jmartino at cancer.rutgers.edu
- TCA Precipitation Problems
Chris Trotta
- Method for RT-PCR?
Jim Hutchins
- test
Tetsuo Shioi
- test
Tetsuo Shioi
- Screening expression library
Manuel Esguerra
- signal transduction pathway
David Tak Yan Yu
- Methods for Ebola virus
R. Dana - Green Gene
- Phosphorylated AP-1 and CREB
rvdeshp0 at INTNET.UPJ.COM
- Problem with Acid gels for basic proteins
Sonya Clark
- Problems with BRL 100 bp ladder
Derek M. Harkins
- corticosterone inplants
Stefano casalotti - ST
- Scal amp resistance gene
John Sargeant
- Experience with resolvase mutation essay?
Christian Beltinger
- Choosing a PCR machine
sabah hussain
- Cheap Photodocumentation
sabah hussain
- Choosing a PCR machine
mhollowa at cris.com
- (none)
RVDESHP$ at PWINET.UPJ.COM
- Customer service.
U12201 at uicvm.uic.edu
- HELP ME TO GET ADD.
bio at ncl.ernet.in
- Cell lines for expression cloning
C. Silvia
- pUCSV3CAT
Alan Cheng
- Is the fusion protein of lambda gt11, ZAP, etc located on the phage surface?
Zhonglin Chai
- Is phage display vector commercially available?
Zhonglin Chai
- Protein expression in LCL by EBV-based vector
TAKAHIKO SAIJO
- What's happened ?
TOUSSAINT Gerthory
- SDS-PAGE HELP
Giorgio Spagnol
- Good WWW site for Molecular Biology Products
fs300627 at sol.yorku.ca
- Reprobing nitrocellulose with protein probes
Petri Kursula
- Crosslinking at acidic pH
Jochen
- Detecting Cu-containing proteins
Jochen
- Promega silver sequencing.
NEAL MUSTO
- Hastings software Co.
Karen Browning
- DNA concentration
Hiranya Roychowdhury
- Protein expression in LCL by EBV-based vector
ab
- looking for protocol for YEAST colony hybridization
borsang at dibit.hsr.it
- Source of Cellophane for Drying Gels
Daniel Kim
- sequencing ssDNA PCR
Marion D. Emerson
- Good Marker for Histiocyte and/or Macrophage ?
Marion Freistadt
- Northern Blot problem
Karen.Straube-West
- Vectors for use in Drosophila S2 cells
Matthew P. Frosch, MD, PhD
- Promega Silver Sequencing
Andrea Johanson
- How to keep the slides for IF.
ynakao at kean.ucs.mun.ca
- fungal identification systems
Juck, David
- Hastings software Co.
Angela Hofstra
- Northern Blot problem
Angela Hofstra
- Northern Blot problem
Angela Hofstra
- Northern Blot problem
Angela Hofstra
- XPOUND Program?
Yun-Long Du, Ph.D.
- REP-PCR primer purity
L. S. del Rosario
- Crosslinking at acidic pH
Andrei Popov
- Subcloning problem
Luis M. Alvarez
- Library Screening Weirdness
schoenfeld at MSSCC.MED.UTAH.EDU
- Molecular Biology Techniques, Tips and Feedback Forum
Anton Scott Goustin
- Cheap Photodocumentation
lees at SASK.USASK.CA
- Genomic DNA isolation
Dr Saleem Mohammed
- protein concentration?
Frank Goetz
- CAT detection
Nigel Kenward
- IN SITU-PCR: looking for advice
Anders Johannisson
- RNA Footprinting
gvacano at gems.vcu.edu
- (none)
Dr. Roberto Staneloni
- PCR reagent decontamination
Lee Smith
- green fluorescent protein (GFP)
Ivica Cerina
- GFP
Ivica Cerina
- CAT detection
Dr I.M. Clark
- Calculate mol. wt, molarity etc from chemical formula
J. A. Kiernan
- growing bacteria in microtite
Nancy Terryn
- (fwd) Good WWW site for Molecular Biology Products
Clemens Suter-Crazzolara
- fungal identification systems
Annick Wilmotte
- plasmid pKC54 sequence needed
Kenneth HOWE
- c5a antibodies -source?
fernanrc at ucbeh.san.uc.edu
- Gentamycin cassettes
Hecht
- Electroporation of Bacteria
Paul N Hengen
- PCR methods needed!
M.C. Fontaine
- fungal identification systems
Annick Wilmotte
- Help Priming with Low Tm primers
Rob Fialho
- REQUEST: isolating histones from cell lysates?
Alberto I Roca
- DNA extraction from plant fossils
Greg Smith
- PCR methods needed!
Fate
- Separating cut from uncut vector
Ira M. Lubin
- DNA/RNA from amphioxus
Sean Stevens
- PCR cloning - PAGE
J. Morrey
- Is phage display vector commercially available?
Zhonglin Chai
- (no subject)
alice tarun
- SYBR Green I
Suzuki Kazuhiko
- Hint in using pGEX for Expression
Evan Burkala
- software mapping plasmid
Annick Wilmotte
- System problem
John Brennand, Tel 4639/5739/6636
- test
Anonymous
- PCR cloning - PAGE
John K. Troyer
- TE saturation of chloroform/isoamyl alcohol?
B VISSER * x2818 Plantkunde *
- Cyanine fluorescent dyes for life science research
Schiller01
- PCR cloning - PAGE
Julian Parkhill
- how about bionet.molbio.pcr?
f.r.byrne
- sequencing services
DEKALB Plant Genetics
- paris
Wen-shyong Tzou
- Best primers for ABI sequencing of Bluescript
RM Brownlie
- PCR Buffer? PARR Buffer?
John J. Cunniff
- PCR cloning - PAGE
Francisco G da Nobrega ib - bio 7588
- GeneReleaser and PCR on Whole Blood
Gary K.
- Plasmid RR1 or RR2
John Sargeant
- pSP64T or other for mRNA/Xenopus Source?
Jody K. Hirsh
- MCP-1
ynakao at kean.ucs.mun.ca
- gel scanning software
Christopher T. Cole
- Hoeffer "minflourometer" Retry?
the End
- test
Sergey V. Orlov
- 380B DNA synthesizer and/or freezing microtome
Jeffrey Chang
- GMP Oligos?
Rebecca Schall
- Primer-dimer problems
Chris Zarow
- glasspowder recipe from FAQ
MAYL at QUCDN.QueensU.CA
- Hoeffer "minflourometer" Retry?
Francisco G da Nobrega ib - bio 7588
- What to use to elute DNA binding protein from DNA ?
S Chen Lab
- Northern blot -- a quick question
Xin Guo
- Really good WWW site for Molecular Biology Products
Joan Boyce
- Wanted: plasmids containing cholera toxin A & B cDNA please
Ian Polkinghorne
- Saturation Mutagenesis protocols request
alice tarun
- Genomic DNA isolation
kang at msvax.mssm.edu
- 380B DNA synthesizer and/or freezing microtome
kang at msvax.mssm.edu
- RNA extraction from Gram negative bacteria
Rosamund Eira Powles
- I'm against molbo.pcr!
Wolfgang Schechinger
- Q: Primer design: Is there a source of statistical base distributions in genomes?
Wolfgang Schechinger
- cDNAs needed !
Clemens Suter-Crazzolara
- (no subject)
Margot Beukers
- Fluorescent labeling of plasmid DNA
Ron Neiswander
- rna 260/280 ratio
Mrs R M D Lastrucci
- Cleaning Glassware
BOIS, DANUTA H
- Integrin blocking antibodies
Vince Morris
- Source of Medium 1065
GORDON BETTS
- blocking Abs for integrins
Vince Morris
- (no subject)
Ron Tate
- (no subject)
Ron Tate
- (no subject)
Ron Tate
- blocking integrin Abs
Vince Morris
- (no subject)
Ron Tate
- (no subject)
Ron Tate
- Help with Growth Media Precipitation
Ron Tate
- Help with Growth Media Precipitation
Ron Tate
- Help with Growth Media Precipitation
Ron Tate
- Is 8 times enough?
Ron Tate
- proteinase K solutions
Howard T. Petrie
- Myosin light chains antibodies
g.coulton
- Mutagenesis
Joanne D. Andreadis
- BA phagemid
Elida at parasit.lan.mcgill.ca
- Best adjustable "ultra-micropipettor"?
the End
- Immunoblotting a calcium-binding protein
Rick Davis
- TE saturation of chloroform/isoamyl alcohol?
Margon Vandongen
- Quantitative PCR
sabah hussain
- HELP:cDNA library from mouse b.marrow/spleen
suihuang at usa1.com
- growing bacteria in microtite
Joanne D. Andreadis
- Help on HPLC and Prostaglandin E2
sjgouldrulesmiggy
- (no subject)
chihara
- cDNA stop codon?
chihara
- Where to go for chromosomal localization of a gene?
ikekim at merle.acns.nwu.edu
- Where to go for in situ hybridization?
ikekim at merle.acns.nwu.edu
- Source of Medium 1065
Brad Nicholson
- Methods Forum
Mark S. Strom
- newly expressed mRNA
David Tak Yan Yu
- Oncogene Science Antibodies??
Robert Fisher
- Quantitative PCR
Thomas Kreuzer
- Gradient maker and collector needed - recommendations?
Shiao Y. Wang
- test
john sinclair
- Freezing damage DNA?
Zhonglin Chai
- transfection of PBMs
john sinclair
- DNA "refusing" to be run in agarose gel
Greg
- Help on HPLC and Prostaglandin E2
Sailesh Surapureddi
- HIS-tag kloning vectors
Margot Beukers
- cleaning elec. cuvettes
Dan Deaguiar
- HIS-tag kloning vectors
Marcel Hoffmann
- DNA "refusing" to be run in agarose gel
Ole Skovgaard
- DNA "refusing" to be run in agarose gel
Adam Greene
- DNA "refusing" to be run in agarose gel
Dan Deaguiar
- Question about your graduate program
Joanne Tornow
- Glass fibers
David Lampe
- CMV promoter leaky in E.Coli?
b.schonhoft
- 4.5 x 9.5 cm Immunodiffusion plates
Elias Klein
- blocking Abs for integrins
dr. jack komisar
- quantitative RT-PCR
Isaac Kim
- DNA "refusing" to be run in agarose gel
Anyname
- InsituPCR to polytenes
pdxrmb at vaxb.nott.ac.uk
- DNA mismatch repair
rvdeshp0 at INTNET.UPJ.COM
- DNA mismatch repair
Donald McDonnell
- Symposia for Industrial Microbiology
Daniel Solaiman
- Non-Specific Ab binding
Anyname
- DNA "refusing" to be run in agarose gel
Ketil Thorstensen
- proteinase K solutions
Rafael Maldonado
- DNA "refusing" to be run in agarose gel
S. L. Rennie
- quantitative RT-PCR
Richard R. Hardy
- Source of Cellophane for Drying Gels
Joan Boyce
- Integrin blocking antibodies/FREE BUYERS GUIDE
Joan Boyce
- DNA "refusing" to be run in agarose gel
Edward E. Diehl
- NIH grant submission template for Mac needed
MALINI at JASON.UTHCT.EDU
- Quantitation of in vitro syn RNA
Chuck Hauser
- Quant of InVitro synthesized RNA
Zeiler, Brian M'bio
- help on PCR from phage plugs
Jennifer Ha x2832
- help on PCR from phage plugs
Jennifer Ha x2832
- Which cDNA library kit?
Amy Marion
- Help! Slooooooow electrophoresis
Keith Hoek -BIOCHEM
- C-terminal cleavage of His-tag
Dr. Jeff Lightfoot
- C-terminal cleavage of His-tag
Joan Boyce
- Can you run an RNA TBE-agarose gel?
jbwatson
- HIS-tag kloning vectors
Frank Chen
- Antigene oligodeoxynucleotide
Kappei KOBAYASHI
- Protein Expression
berns001 at coyote.csusm.edu
- Membrane protein expressed in Baculovirus?
mhollowa at cris.com
- effect of '3 UTR on recombinant expression
Niels Groot
- ALF Express Problems
Douglas Buechter
- lambdaZAP massexcision
Adriana
- Computer Virus from American Cancer Society diskette
Young Whang
- ANTIBODIES TO CALMODULIN
dilip
- Protein Expression
brett
- óan you run an RNA TBE-agarose gel
Sergey V. Orlov
- effect of 3' UTR on recombinant expression
Sergey V. Orlov
- Phage displayed cDNA libs.
Troels Wind
- HELP! DNA from foetal tissue
frances at matai.vuw.ac.nz
- does this work?
brian decker
- Requesting apple genomic library
Luis Destefano Beltran
- Help! Slooooooow electrophoresis
skim at ALLIANT.SNU.AC.KR
- Setting up a lab at home
Susan Jane Hogarth
- DNA "refusing" to be run in agarose gel
Bixing Huang
- Help with PLP mutagenesis
Mary_Wood at BAYLOR.EDU
- Setting up a lab at hom
"Hansen Lab", not U.H.
- LR44 Batteries
David J. Gerrick
- NG108-15 cell line
PLONKSG at ctrvax.Vanderbilt.Edu
- Cleaning glassware for DNA
Danuta Bois
- Error rate in Deng/Nickoloff mutagenesi
Scott Vande Pol
- B-Gal Staining
jg
- Beta Gal Transfection Assay
Dr. L. Fliegel
- direct PCR amplifiction using library
dinakar bhattramakki
- Help: Can't see biotin:riboprobes in Agarose gels
J S Abrams
- Why? In vivo exsicion didn't work.
Mari L. Shinohara
- direct PCR amplification using library
Sergey V. Orlov
- Deng/Nickoloff mutagenesis
Scott Vande Pol
- restrict. digestion of PCR products
sl3wx at cc.usu.edu
- Western Blot
Alison Weaver
- Non-essential regions of B-lactamase gene?
andrecp at perkin-elmer.com
- direct PCR amplification using library
To: Nikolai
- Northern blot -- a quick question
Joan Boyce
- Is there something wrong???
Shahram Mori
- Northern blots--lymphocyte RNA
Cathy Sprankle
- TnPhoA mutagenesis question
cliff at molbiol.uct.ac.za
- EST sequencing strategy
David Adelson
- prepare DNA from flowering plant Azalea (rhododendron, Ericaceae)
Nancy Terryn
- help: crosslinking throug -COOH group
Hun-Sik KIM, M.D.
- Western Blot: HELP!!!!
Klaus Lehnert
- PDQUEST for Mac?
Christer Ericsson
- Western Blot
skim at ALLIANT.SNU.AC.KR
- Help: Characterization of Jurkat T-cell line
Volker Blaschke
- Statistical programs
Gregory M. Acland
- EtoH ppt.
DYDM6025 at BUREAU.UCC.IE
- help with pcr cloning
Sailesh Surapureddi
- help with pcr cloning
Sailesh Surapureddi
- rna storage
brad schonhoft
- Best primers for ABI sequencing of Bluescript
(Ian Ridgers)
- Electroporation of Bacteria
Joan Boyce
- LacSwitch Inducible Mammalian Expression System
Tim Lansing
- need human endometrial cell line MD-E
Chuck Miller
- Help: Characterization of Jurkat T-cell line
mary karczewski
- rna storage
Jillian Johnston
- protein elution
Tomas Drgon
- Floureporter to detect LacZ
john sinclair
- Floureporter to detect LacZ
john sinclair
- HELP, LOCATE ANTIBODIES FOR CALMODULIN
DILIP DIAS
- cDNA Library
N490047 at UNIVSCVM.CSD.SCAROLINA.EDU
- Prestaining DNA with SYBR Green I.
Paul St. Amand
- Antibody for His-Tag
Jim Beasley
- LacSwitch Inducible Mammalian Expression System
Kenneth Y. Ilio
- Does RNasin inhibit RT?
Steven Sullivan
- Problem with fluorescein
Serguei Golovan
- MS Conference Announcement
irc at III.NET
- Antibody for His-Tag
Frank Chen
- Ampicillin resistance screening.
Stefan Albert Bledig
- Copies of Journal articles
Santhat Lekhavadthanajai - SCMI - 3636187
- ubiquinone midpoint potential at neutral pH in aqueous medium?
E. C. Nelson
- propidium iodide staining to measure apoptosis?
anmab2 at orion.alaska.edu
- san you run an RNA TBE-agarose gel
Sergey V. Orlov
- Wistar Inst., U-Penn.
Troels Wind
- MNNG: WHAT DOES IT DISSOLVE IN?
Mr
- EtoH ppt
DYDM6025 at BUREAU.UCC.IE
- help: primer restriction sites
William Alexander
- APO B 48 ANTISERA(ANY SPECIES)
Henry Wilcox
- PCR Lab Design
Jeremy Carson
- HLA-DQ expression
shethah morgan
- help: primer restriction sites
Frank Chen
- 2D-PAGE
Jeney Csaba
- primer restriction sites
Andrei Popov
- microtiterplates for sequencing
m.a.jones
- Non-radioactive sequencing
sljj5 at cc.usu.edu
- help: primer restriction sites
Rebecca Schall
- Help: stigma type and pollen number
Ping Li
- Electroelution/Centricon Problem
Barry Moore
- Green Fluorescent Protein cDNA needed
Yves HATZFELD
- Help on site-directed mutagenesis kit
Eric Trottier
- Help on sequencing appartus
Eric Trottier
- Enzyme with 5'to 3' ssDNA exonuclease activity?
Juergen Sasse (Path)
- Camcorder/Photodocumentation
Tim Uphoff
- Vero cells transfection/internal control
Alexandra Martins
- flow sorting plant chromosomes
David S. Guttman
- Camcorder/Photodocumentation
Tim Uphoff
- Etoh ppt
Michael Romero
- help: crosslinking...
Donna Smith
- Quantitative PCR
James Scutero
- Unstable Construct
CHENJF at HELIX.MGH.HARVARD.EDU
- 12CA5 MAb immunoprecipitation
Jean-Yves Masson
- How to modify QuickTake100 camera for gel documentation?
The Wagner Lab
- software and board for photo-documentations.
Frank Chen
- In situ PCR
John Bussell
- In situ PCR
John Bussell
- ?GC rich sequencing on ABI377.
- NEED quick n easy chaperone Assays
SAIER LAB
- SEQUENCING Large Plasmids
Steve Wylie
- Setting up a lab at home
kang at msvax.mssm.edu
- ANNOUNCEMENT: have you a protein to predict? FINAL Call for
prediction targets
Tim Hubbard
- HELP:cloning into pGEM-T
Berthold Bettenhausen
- Random mutagenesis
RBurnap
- mutagenesis kit
Arturo Garcia-Horsman
- Mouse strain 129 DNA
Todd Miller - Pharmacology
- subtractive hybridisation
Debbie
- COMET ASSAYS
g.coulton
- in situ/fixatives
Phillip c. Hartig
- 12CA5 MAb immunoprecipitation
brett
- ABI 310 prism
Mike Dalrymple
- yeast chromosomal DNA digest
Andrei Popov
- STRONG promotor
Marc Van de Craen
- Test
David Chia
- Ab synth in bacteriophage
Michael Myers
- Elutrap????
Peter
- PCR problem
David Chia
- Source for gDNA across phyla?
Michael Myers
- PCR primer instability
Mark Naylor
- Quantitative PCR
Frank Chen
- Help! transfection of ME-180 cells
Gary Brown
- software and board for photo-documentations.
Joan Boyce
- Setting up a lab at home
Sean Eddy
- Carnivorous Plants-Tiss Cult-A tumefaciens
Garry P. Nolan
- Owl Scientific
Banstead at aol.com
- need help map pCis
Melissa
- citation software
Daniel,R,Chagnovich
- Plasmid drawing software?
bicfo at flinders.edu.au
- Ca Phosphate Transfection/HUVEC Cells
Christopher Wong
- M13: plates are all blue!
Jo Caine
- Molecular Biology Products
Joanna Aretz
- fluorography?
Xin Guo
- Yeast chromosomal EcoRI digests
paul bundock
- Quant. RT-PCR: continued
suihuang at usa1.com
- Sorvall RC-24 Centrifuge
user at ichr.uwa.edu.au
- fluorography?
NICHOLAS THEODORAKIS
Last message date:
Thu Aug 31 22:29:13 EST 1995
Archived on: Sun Mar 6 20:42:19 EST 2005
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