ECL reagents

[iddavis at vms.cis.pitt.edu]

In article <mit.edu-3011951527540001 at cancer-ctr-mac-32.mit.edu>, mit.edu (k) 
writes:
> Does anyone know if it is possible to dilute the ECL reagents (from
> Amersham) and still get adequate detection?  If so what do you dilute them
> in?  Thanks

So, we meet again....  8^}

Yes, I used to dilute them in DDW routinely, 1:5 (ie 4 ml DDW, 0.5 ml
each ECL component).  Worked fine.

Ian Davis				iddavis at vms.cis.pitt.edu