RediVue and EasyTides..Good?

Peter Thorén peter.thoren at genetik.uu.se
Fri Dec 1 03:12:43 EST 1995


In article <48sngd$7l7 at reeve.research.aa.wl.com> Angela Hofstra <Hofstra at aa.wl.com> writes:
>From: Angela Hofstra <Hofstra at aa.wl.com>
>Subject: Re: RediVue and EasyTides..Good?
>Date: 21 Nov 1995 14:25:17 GMT

>tflanigan at molbiol.ox.ac.uk wrote:
>stuff deleted

>>Tom,
>>we've looked at using redivue and easytides in differential display of mRNA
>>and they did not work.  The preservative(?) seems to interfere with the PCR 
>>reaction.  We were also told that these nucleotides did not work well for in 
>>situ hybridisation but we did not try it.
>>Cheers,
>>Tom Flanigan

>Thanks for this tip. We were planning to use redivue nucleotides in PCR 
>and now we'll reconsider.

>Angela


Dear Angela,

In our lab we have been using redivue S35dATP in our PCR reactions for a long 
time . Because of the hazard with using S35 in a PCR reaction we have now 
switched to redivue P33dATP wich also gives excellent results!

Best wishes,

Peter Thorén
Dept.of Genetics, Uppsala University
peter.thoren at genetik.uu.se
------------------------------------------------------------
Peter Thorén, Dept.of Genetics, Uppsala University
Box 7003, S-750 07 Uppsala, Sweden, phone: +46 18 67 26 61
e-mail: peter.thoren at genetik.uu.se, FAX +46 18 67 27 05



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