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Ethidium Bromide question

Morrison Lab fergusb at microbio.lifesci.ucla.edu
Fri Dec 1 14:59:40 EST 1995


IT RUNS IN THE OPPOSITE DIRECTION - EASY IF YOU THINK ABOUT IT - DNA IS
NEGATIVELY CHARGED SO THE ET.BR. BINDS TO IT AS IT IS POSITIVELY CHARGED
SO THE ET. BR. RUNS IN THE OPPOSITE DIRECTION TO THE DNA - IF YOU PUT IT
IN THE AGAROSE MIX - THEN YOU GET THOSE TWO TONE GELS - YOU CAN OVERCOME
THIS BY PUTTING THE ET.BR. IN THE RUNNING BUFFER

FERGUS
UCLA

In article <hopkinsc.13.000D9B7A at lincoln.ac.nz>, hopkinsc at lincoln.ac.nz
(Hopkins, Charlotte) wrote:

> I am trying to find out which way Ethidium bromide runs in an agrose gel.  Is 
> it in the same direction as the DNA (negative to positive) or in the opposite 
> direction
> My email address is hopkinsc at tui.lincoln.ac.nz
> 
> Many thanks for any advice you may have
> 
> Charlotte Cameron
> Lincoln University
> Canterbury
> New Zealand



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