In article <30BD8A2C.58C9 at medkem.umu.se>, Eric Hanson
<Eric.Hansson at medkem.umu.se> wrote:
When I've done gel shift experiments with whole yeast cell extracts I will
first add the "cold" competitor DNA to the reacation containing the yeast
extract, incubate for 15 minutes--I then add the labeled DNA, incubate for
an additional 15 minutes and then load the gel.