fresh APS (former SDS-PAGE)

[Stephen R. Lasky]

In article <voeller-0212950659470001 at voeller.his.com>, voeller at his.com
(Jim Voeller) wrote:

> If I can add something here. I know that every source says to use fresh
> APS, but......I make 10% APS and freeze single use aliquots at -20 and it
> works forever! Well I haven't tested the forever part but it works for at
> least 6 months. Try it.
> 
> -- 
> Jim Voeller
> voellerj at gunet.georgetown.edu
> Lombardi Cancer Center, Georgetown University
> Washington DC, USA

Jim, you've been lucky.  When I was a grad student I tried doing the same
thing you describe.  After a run of failed gels just when I was trying to
finish off a paper, I traced the problem back to the APS.  I make it fresh
every time now and teach my students and postdocs to do the same.  After
all, how hard is it to make a 10% APS solution?  Dump some APS out an a
weigh paper, see what it weighs, add enough water to make 10% and mix. 
Takes about as long as defrosting a tube of frozen APS.

SRLasky

-- 
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Stephen R. Lasky Ph.D.   Brown U/Roger Williams Medical Center,  Providence, RI.   
Phone: 401-456-5672     Fax: 401-456-6569     e:mail: Stephen_Lasky at brown.edu
===================================================================
America may be unique in being a country which has leapt from barbarism to decadence without touching civilization.  John O'Hara.
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^