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RT-PCR vs Northern

Stephen R. Lasky Stephen_Lasky at brown.edu
Sat Dec 2 06:41:39 EST 1995


In article <49l20s$bq at mark.ucdavis.edu>, ez008413 at bullwinkle.ucdavis.edu
(Gabriel Romero) wrote:

> I'm about to start gene expression studies at the transcription level but 
> I have not decided between RT-PCR and Northern analysis as the method to 
> use.  I only need to have a yes or no answer for expression initially and 
> then have some idea on the level of expression.  How do the methods 
> compare in terms of ease/difficulty in running the experiments, and in 
> terms of amount and quality of information?
> 
> Thanks for any advice.
> 
> 
> Gabby Romero

RT-PCR is much more sensitive, faster, requires less RNA, and is easy to
do.  It is, however, more difficult to quantitate.  You also need to be
able to design good primers (which is usually not too much of a problem).

SRLasky

-- 
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Stephen R. Lasky Ph.D.   Brown U/Roger Williams Medical Center,  Providence, RI.   
Phone: 401-456-5672     Fax: 401-456-6569     e:mail: Stephen_Lasky at brown.edu
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America may be unique in being a country which has leapt from barbarism to decadence without touching civilization.  John O'Hara.
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