fresh APS (former SDS-PAGE)
RMCMAHON at ollamh.ucd.ie
Sun Dec 3 12:12:54 EST 1995
> Forwarded by: server-daemon <server-daemon at dl.ac.uk>
> Date sent: Sat, 02 Dec 1995 07:46:17 -0400
> Date forwarded: Sat, 2 Dec 95 15:38:49 UT
> From: Stephen_Lasky at brown.edu (Stephen R. Lasky)
> Send reply to: Stephen_Lasky at brown.edu (Stephen R. Lasky)
> To: "bionet.molbio.methds-reagnts mail newsgroup" <bionet-news at dl.ac.uk>
> Subject: Re: fresh APS (former SDS-PAGE)
> In article <voeller-0212950659470001 at voeller.his.com>, voeller at his.com
> (Jim Voeller) wrote:
> > If I can add something here. I know that every source says to use fresh
> > APS, but......I make 10% APS and freeze single use aliquots at -20 and it
> > works forever! Well I haven't tested the forever part but it works for at
> > least 6 months. Try it.
> > --
> > Jim Voeller
> > voellerj at gunet.georgetown.edu
> > Lombardi Cancer Center, Georgetown University
> > Washington DC, USA
> Jim, you've been lucky. When I was a grad student I tried doing the same
> thing you describe. After a run of failed gels just when I was trying to
> finish off a paper, I traced the problem back to the APS. I make it fresh
> every time now and teach my students and postdocs to do the same. After
> all, how hard is it to make a 10% APS solution? Dump some APS out an a
> weigh paper, see what it weighs, add enough water to make 10% and mix.
> Takes about as long as defrosting a tube of frozen APS.
> Stephen R. Lasky Ph.D. Brown U/Roger Williams Medical Center, Providence, RI.
> Phone: 401-456-5672 Fax: 401-456-6569 e:mail: Stephen_Lasky at brown.edu
> America may be unique in being a country which has leapt from barbarism to decadence without touching civilization. John O'Hara.
Just to support Jim, I make up a 20% solution of APS and store it in
the fridge. I have never had a problem with any of my gels.
More information about the Methods