fresh APS (former SDS-PAGE)

RUTH MCMAHON RMCMAHON at ollamh.ucd.ie
Sun Dec 3 12:12:54 EST 1995


> Forwarded by:   server-daemon <server-daemon at dl.ac.uk>
> Date sent:      Sat, 02 Dec 1995 07:46:17 -0400
> Date forwarded: Sat, 2 Dec 95 15:38:49 UT
> From:           Stephen_Lasky at brown.edu (Stephen R. Lasky)
> Send reply to:  Stephen_Lasky at brown.edu (Stephen R. Lasky)
> To:             "bionet.molbio.methds-reagnts mail newsgroup" <bionet-news at dl.ac.uk>
> Subject:        Re: fresh APS (former SDS-PAGE)

> In article <voeller-0212950659470001 at voeller.his.com>, voeller at his.com
> (Jim Voeller) wrote:
> 
> > If I can add something here. I know that every source says to use fresh
> > APS, but......I make 10% APS and freeze single use aliquots at -20 and it
> > works forever! Well I haven't tested the forever part but it works for at
> > least 6 months. Try it.
> > 
> > -- 
> > Jim Voeller
> > voellerj at gunet.georgetown.edu
> > Lombardi Cancer Center, Georgetown University
> > Washington DC, USA
> 
> Jim, you've been lucky.  When I was a grad student I tried doing the same
> thing you describe.  After a run of failed gels just when I was trying to
> finish off a paper, I traced the problem back to the APS.  I make it fresh
> every time now and teach my students and postdocs to do the same.  After
> all, how hard is it to make a 10% APS solution?  Dump some APS out an a
> weigh paper, see what it weighs, add enough water to make 10% and mix. 
> Takes about as long as defrosting a tube of frozen APS.
> 
> SRLasky
> 
> -- 
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> Stephen R. Lasky Ph.D.   Brown U/Roger Williams Medical Center,  Providence, RI.   
> Phone: 401-456-5672     Fax: 401-456-6569     e:mail: Stephen_Lasky at brown.edu
> ===================================================================
> America may be unique in being a country which has leapt from barbarism to decadence without touching civilization.  John O'Hara.
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> 

Just to support Jim, I make up a 20% solution of APS and store it in 
the fridge. I have never had a problem with any of my gels.
Ruth. 



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