IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Ultracompetent E.coli cells?

Stephan Witte stephan.witte at uni-konstanz.de
Mon Dec 4 03:57:01 EST 1995


I«m looking for highly competent E.coli cells for a library transformation.
Our homemade CaCl2-competent  cells yield app. 5E4  transformants per ug
DNA (6 kb plasmid)
I think, this is not much.
Are there better protocols for preparing highly competent coli-cells?
What to think about Stratagenes ultracompetent cells? What«s the trick?

thanks in advance


Stephan Witte
Inst. of Immunology
University of Constance
Postfach 5560 M 660
78434 Konstanz

Phone: +49-7531-88-2749
Fax:     +49-7531-88-3102
e-mail: stephan.witte at uni-konstanz.de

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net