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Ultracompetent E.coli cells?

Stephan Witte stephan.witte at uni-konstanz.de
Mon Dec 4 03:57:01 EST 1995


Hi,

I«m looking for highly competent E.coli cells for a library transformation.
Our homemade CaCl2-competent  cells yield app. 5E4  transformants per ug
DNA (6 kb plasmid)
I think, this is not much.
Are there better protocols for preparing highly competent coli-cells?
What to think about Stratagenes ultracompetent cells? What«s the trick?

thanks in advance

Stephan




*******************************************
Stephan Witte
Inst. of Immunology
University of Constance
Postfach 5560 M 660
78434 Konstanz
Germany

Phone: +49-7531-88-2749
Fax:     +49-7531-88-3102
e-mail: stephan.witte at uni-konstanz.de
*******************************************



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