fresh APS (former SDS-PAGE)

Chris Greene cgreene at bimcore.emory.edu
Mon Dec 4 23:42:05 EST 1995


Stephen R. Lasky (Stephen_Lasky at brown.edu) wrote:
: In article <voeller-0212950659470001 at voeller.his.com>, voeller at his.com
: (Jim Voeller) wrote:

: > If I can add something here. I know that every source says to use fresh
: > APS, but......I make 10% APS and freeze single use aliquots at -20 and it
: > works forever! Well I haven't tested the forever part but it works for at
: > least 6 months. Try it.
: > 
: > -- 
: > Jim Voeller
: > voellerj at gunet.georgetown.edu
: > Lombardi Cancer Center, Georgetown University
: > Washington DC, USA

: Jim, you've been lucky.  When I was a grad student I tried doing the same
: thing you describe.  After a run of failed gels just when I was trying to
: finish off a paper, I traced the problem back to the APS.  I make it fresh
: every time now and teach my students and postdocs to do the same.  After
: all, how hard is it to make a 10% APS solution?  Dump some APS out an a
: weigh paper, see what it weighs, add enough water to make 10% and mix. 
: Takes about as long as defrosting a tube of frozen APS.

: SRLasky

: -- 
: ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
: Stephen R. Lasky Ph.D.   Brown U/Roger Williams Medical Center,  Providence, RI.   
: Phone: 401-456-5672     Fax: 401-456-6569     e:mail: Stephen_Lasky at brown.edu
: ===================================================================
: America may be unique in being a country which has leapt from barbarism to decadence without touching civilization.  John O'Hara.
: ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^

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