Does anyone have experience working with B. subtilis L-forms?
1) Inducing the bacteria into the L-form,
2) Transformation of L-forms (I think they will behave as super-
competent cells?), and
3) Expression of genes on plasmids in the B. subtilis L-form.
Are there any special considerations necessary in plasmids for use
in B. subtilis? Or can I just use an expression vector that has
been designed for use in E. coli.
Any help or advice that anyone can give me would be greatly
My address is:
hugp at box-h.nih.gov
9000 Rockville Pike
Building 10, Room 4A01
Bethesda, MD 20891