Designing in restriction sites

[Rick Neubig]

Hello,
I am interested in designing unique restriction sites into a particular
general area of a plasmid without changing the expressed protein. There
are no naturally occuring restriction sites. Two questions:

1) Is there software that tackles this kind of problem or are there
general approaches which have been taken to simplify this?

2) Is there a good place to get a list of restriction enzymes
sorted by their recognition sequence (not by enzyme name)? This
would help me with this process.

Thanks in advance,
Rick
_________________________________________________________
Rick Neubig                             RNeubig at umich.edu
Pharmacology
University of Michigan               Phone (313) 763-3650
http://www.umich.edu/~rneubig        FAX   (313) 763-4450