Designing in restriction sites

Rick Neubig RNeubig at
Tue Dec 5 18:22:46 EST 1995

I am interested in designing unique restriction sites into a particular
general area of a plasmid without changing the expressed protein. There
are no naturally occuring restriction sites. Two questions:

1) Is there software that tackles this kind of problem or are there
general approaches which have been taken to simplify this?

2) Is there a good place to get a list of restriction enzymes
sorted by their recognition sequence (not by enzyme name)? This
would help me with this process.

Thanks in advance,
Rick Neubig                             RNeubig at
University of Michigan               Phone (313) 763-3650        FAX   (313) 763-4450

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