I am attempting to transfect 293 cells with large ~40kb plasmid carrying the
adenovirus whole genome, using DOTAP. I dont seem to get good efficiency of
transfection. Also I get a high cell death, (DOTAP-DNA-media mixture is kept for
overnight on the cells). Can anyne having experience with lipofecting large plasmids
help me out with this.
Obaid Yusuf Khan