In article <49d4q2$fg5 at nnrp.ucs.ubc.ca>, lpss at unixg.ubc.ca (Alex Chang) says:
>>Something wired happened to my SDS-PAGE gel. I did SDS-PAGE many times
>before, but haven't done it for a while. Recently, when I adapted my old
>protocal for a mini-gel, it takes hours for the separating gel to
>solidify and even worse, the stacking gel solidified poorly and the wells
>did not contain the samples very well as the result of it.
>University of British Columbia
>achang at hivnet.ubc.ca
Do you use the same old lot of ammonium persulfate? If yes, a suggest
you change to a new one. In my hand ammonium persulfate, when improperly
stored, become hydrated and in this form is unable to catalize acrylamide
Joab Trajano Silva.