Circular DNA & EthBr in electrophoresis

Bart Frank frank at omrf
Tue Dec 5 10:01:05 EST 1995


Can someone refer me to data on the migration of circular molecules
in agarose gels with and without the gels being prestained with
ethidium bromide?  We see a series of bands of slightly differing
size when a 2 kb insert is ligated to itself in gels stained 
after electrophoresis, but a predominately stained band
if we place similarly treated samples in empty lanes of gels which
had previously been stained before (& then after electrophoresis).
Is this an expected anomaly?
Thanks,
Bart Frank



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