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magnesium in pcr - what does it do?

Daniel Mytelka mytelka at zenith.Berkeley.EDU
Tue Dec 5 15:58:56 EST 1995


The actual substrate for polymerization is dNTP-Mg++. Mg++
co-ordinates between charges on two of the phosphate groups,
and stabilizes contacts in the polymerase active site. 
Current theory is that there are actually two divalent
cations necessary for polymerization, though the second one
may not have to be Mg++.

DSM




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