In article <4a0fsu$nkr at itssrv1.ucsf.edu>, bgold at itsa.ucsf.edu (Bert Gold) wrote:
> My conditions are: 95 degrees for 2 mins.
>> Then, 10X 95 degress for 45 secs.
> 60 degrees for 1 minute
> 68 degrees for 4 mins.
Is there any particular reason to use 68 degrees for extension in long PCR
instead of 72 degrees? I used 72 deg for amplification 0f 2.5 kb DNA
fragment using the same BMB kit (1yr old). It worked fine.
Just a thought.