3.2 Kb PCR Problems

Mikhail Alexeyev malexeyev at biost1.thi.tmc.edu
Tue Dec 5 11:22:34 EST 1995


In article <4a0fsu$nkr at itssrv1.ucsf.edu>, bgold at itsa.ucsf.edu (Bert Gold) wrote:

> My conditions are:  95 degrees for 2 mins.
>                
>    Then, 10X        95 degress for 45 secs.
>                     60 degrees for 1 minute
>                     68 degrees for 4 mins.
> 
 
Is there any particular reason to use 68 degrees for extension in long PCR
instead of 72 degrees? I used 72 deg for amplification 0f 2.5 kb DNA
fragment using the same BMB kit (1yr old). It worked fine.

Just a thought.

M. Alexeyev



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