PCR product cleanup for sequencing...

Bill Burnett bbur at wpo.nerc.ac.uk
Tue Dec 5 14:41:51 EST 1995


Hi All...

I'm just about to start automated sequencing of a 1500bp PCR product, with an 
ABI dye terminator kit...
What's the best way to clean up the PCR's?
I've used Promega Wizard preps before which worked okay...  but that was a 
while ago when ABI were recommending them... now they tell me the wizards are 
no good and I should be using Microcon-100's..........  The folks with the 
auto-sequencer say stick with the wizards...

Anyone want to vote?
I'll probably be trying both seeing as I have them...

Bill
----------------------------------------------------
Bill Burnett - bbur at wpo.nerc.ac.uk
Scottish Association for Marine Science
P.O. Box 3, Oban, Argyll PA34 4AD, UK



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