Bacillus subtilis L-forms

["Marianne Leverone ", BIO]

hugp:  will the expression vector you want to use replicate in Bacillus?


On Mon, 4 Dec 1995 hugp at nih.gov wrote:

> Does anyone have experience working with B. subtilis L-forms?
> 
> Specifically:
> 
> 1)  Inducing the bacteria into the L-form,
> 
> 2)  Transformation of L-forms (I think they will behave as super-
>     competent cells?), and
> 
> 3)  Expression of genes on plasmids in the B. subtilis L-form.
> 
> Are there any special considerations necessary in plasmids for use
> in B. subtilis?  Or can I just use an expression vector that has 
> been designed for use in E. coli.
> 
> Any help or advice that anyone can give me would be greatly 
> appreciated.
> 
> My address is:
> 
> hugp at box-h.nih.gov
> (or)
> Peter Hug
> NIH
> 9000 Rockville Pike
> Building 10, Room 4A01
> Bethesda, MD 20891
> 
> (301) 496-8832
> 
> Thanks!
> 
>