hugp: will the expression vector you want to use replicate in Bacillus?
On Mon, 4 Dec 1995 hugp at nih.gov wrote:
> Does anyone have experience working with B. subtilis L-forms?
>> 1) Inducing the bacteria into the L-form,
>> 2) Transformation of L-forms (I think they will behave as super-
> competent cells?), and
>> 3) Expression of genes on plasmids in the B. subtilis L-form.
>> Are there any special considerations necessary in plasmids for use
> in B. subtilis? Or can I just use an expression vector that has
> been designed for use in E. coli.
>> Any help or advice that anyone can give me would be greatly
>> My address is:
>>hugp at box-h.nih.gov> (or)
> Peter Hug
> 9000 Rockville Pike
> Building 10, Room 4A01
> Bethesda, MD 20891
>> (301) 496-8832