Two-hybrid question

Steven Loechel frosty at biobase.dk
Tue Dec 5 02:43:50 EST 1995


I've been using the two-hybrid system to screen cDNA libraries, and 
have gotten reasonable but not totally satisfying results with the
vector pAS2 and the yeast strain CG1945, purchased from Clontech.

Now Clontech is pushing pAS2-1 and Y190.  Does anyone have enough
experience with these various vectors and yeast strains to offer 
advice as to their advantages and drawbacks?


Regards,
Frosty Loechel



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