we are actually sequencing a long size fragment of DNA, from a cosmid
library. We have several problems. I hope we could find help here.
Is the size of the vector (plasmid) in relation with the size of the DNA
What plasmid could we use to insert a 15kb DNA fragment ? (I said long size)
What is the ideal transfection methods for such a plasmid, and in which
bacteria could we transfected it ?
Thanks for e-mailed answers.