Long size fragments cloning

Luc Ferrari ferrari at ctrmed.u-nancy.fr
Tue Dec 5 05:10:52 EST 1995


Hi,

we are actually sequencing a long size fragment of DNA, from a cosmid 
library. We have several problems. I hope we could find help here.

Is the size of the vector (plasmid) in relation with the size of the DNA 
insert ?

What plasmid could we use to insert a 15kb DNA fragment ? (I said long size)

What is the ideal transfection methods for such a plasmid, and in which 
bacteria could we transfected it ?

Thanks for e-mailed answers.

Luc




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