Stripping blots

cok at mole.bio.cam.ac.uk c.okane at gen.cam.ac.uk
Tue Dec 5 04:53:28 EST 1995


Hi,
   We have a few valuable blots, mainly hybond-N, with DNA bound. What's
the best way to strip off a probe with minimum wear and tear to the blot
and the immobilised DNA? We use DIG-labelled probes, so the stripping has
to work well too, because we can't just wait for the probe to decay.

Thanks,
Cahir
cok at mole.bio.cam.ac.uk

-- 
Cahir O'Kane, Dept of Genetics, Univ. of Cambridge, UK
cok at mole.bio.cam.ac.uk



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