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Stripping blots

cok at mole.bio.cam.ac.uk c.okane at gen.cam.ac.uk
Tue Dec 5 04:53:28 EST 1995

   We have a few valuable blots, mainly hybond-N, with DNA bound. What's
the best way to strip off a probe with minimum wear and tear to the blot
and the immobilised DNA? We use DIG-labelled probes, so the stripping has
to work well too, because we can't just wait for the probe to decay.

cok at mole.bio.cam.ac.uk

Cahir O'Kane, Dept of Genetics, Univ. of Cambridge, UK
cok at mole.bio.cam.ac.uk

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