transformation of E. coli SM10

Mikhail Alexeyev malexeyev at biost1.thi.tmc.edu
Tue Dec 5 20:09:30 EST 1995


In article <T.Barnett-0612951111450001 at 131.217.101.135>,
T.Barnett at path.utas.edu.au (Tim Barnett) wrote:

> I am trying to transform E. coli SM10 or S17-1, both of which possess the
> RP4 transfer regions in trans necessary for subsequent mobilisation of
> plasmids via conjugation. 

Hi, Tim:

I don't have much experience with S17-1, but I was able to obtain up to
10^7 cfu/ug S17-1/lambda pir using both Hanahan procedure and procedure
described in Chung, Miller Meth. Enzymol. v 218 p.621. I stick to the
latter one 'cos it simple and quite reproducible.

Good luck,
M.A.



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