Ultracompetent E.coli cells?

jpv at cayman.necker.fr jpv at cayman.necker.fr
Wed Dec 6 12:43:20 EST 1995


In article <stephan.witte-041295105947 at marvin.biologie.uni-konstanz.de>, 
<stephan.witte at uni-konstanz.de> writes:
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> From: stephan.witte at uni-konstanz.de (Stephan Witte)
> Newsgroups: bionet.molbio.methds-reagnts
> Subject: Ultracompetent E.coli cells?
> Followup-To: bionet.molbio.methds-reagnts
> Date: 4 Dec 1995 08:57:01 GMT
> Organization: University of Constance
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> Message-ID: <stephan.witte-041295105947 at marvin.biologie.uni-konstanz.de>
> NNTP-Posting-Host: marvin.biologie.uni-konstanz.de
> 
> Hi,
> 
> I«m looking for highly competent E.coli cells for a library transformation.
> Our homemade CaCl2-competent  cells yield app. 5E4  transformants per ug
> DNA (6 kb plasmid)
> I think, this is not much.
> Are there better protocols for preparing highly competent coli-cells?
> What to think about Stratagenes ultracompetent cells? What«s the trick?
> 
> thanks in advance
> 
> Stephan
> 
> 
> 
> 
> *******************************************
> Stephan Witte
> Inst. of Immunology
> University of Constance
> Postfach 5560 M 660
> 78434 Konstanz
> Germany
> 
> Phone: +49-7531-88-2749
> Fax:     +49-7531-88-3102
> e-mail: stephan.witte at uni-konstanz.de
> *******************************************


Electroporation is probably the best way to get ultra-yields (5 E9/ng)



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