Efficient expression shuttle vectors derived from RSF1010 with promoters
working efficiently in both Pseudomonads and E. coli have been described
Bagdasarian et al., Gene 26:273-282 (1983)
Frey et al., Gene 62:237-247 (1988)
Frey,J. Res.Microbiol. 143:263-269 (1992)
If you are looking for some of them, please feel free to contact me.
chend at ucs.orst.edu (Don Chen) wrote:
>>I don't know if my previous post made it in, so here's my question
>>Does anyone have information regarding cloning vectors for Pseudomonas
>spp that can also work in Ecoli? The type of vectors include, but not
>restricted to, pBR-type cloning vectors, shuttle vectors, vectors with
>transposon cassettes, and transducing phage vectors. I understand
>that Pseudomonas promoters are not, or poorly, recognized inside of
>Ecoli - is there a way around this, maybe one with an Ecoli promoter
>in frame with the cloning site? Thanks for any help.