20kb Long PCR and nucleotide source

Duncan Clark Duncan at genesys.demon.co.uk
Wed Dec 6 09:36:10 EST 1995


Hi Folks,

If anyone is routinely performing long PCR of greater than 20kb can you let me 
know whose nucleotides you are using? I find that up to 15kb it doesn't matter 
but beyond that (ie 20kb) the nucleotide source is critical.

Many thanks

Duncan 
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