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homemade T addition or blut PCR cloning?

Catherine Raven craven at molbiol.ox.ac.uk
Thu Dec 7 13:38:25 EST 1995

2) or 4) but see previous reply on newsgroup regarding cutting near 
termini of PCR, and NEB page 208 regarding enzymes which don't like 
doing this. I have done a lot of 4) but would revert to two if I had 
problems, although you may get more false positives.


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