pseudomonas vectors

Tim Barnett T.Barnett at
Thu Dec 7 19:50:19 EST 1995

 Don Chen wrote:
> Hi:
> I don't know if my previous post made it in, so here's my question
> again.
> Does anyone have information regarding cloning vectors for Pseudomonas
> spp that can also work in Ecoli?  The type of vectors include, but not
> restricted to, pBR-type cloning vectors, shuttle vectors, vectors with
> transposon cassettes, and transducing phage vectors.  I understand
> that Pseudomonas promoters are not, or poorly, recognized inside of
> Ecoli - is there a way around this, maybe one with an Ecoli promoter
> in frame with the cloning site?  Thanks for any help.
> Don

pUCP19 (AmpR) is an E. coli-Pseudomonas shuttle vector and is described in
West et al. (1994) Gene 148: 81-86.  There is also the pBBR1MCS series of
plasmids which replicate in Pseudomonas.  These are a series 5 plasmids
encoding resistance to one of Cml, Kan, Tet, Amp or Gent and were first
described by Kovach et al. (1994) Biotechniques 16: 800-802 and later in
Gene 161: 175-176.

Both of these have a MCS in the lacZalpha gene which alows blue/white
selection in suitable hosts.  The pBBR1MCS series also have a mob site
which alows conjugal transfer when the RP4 transfer functions are provided
in trans.  

Best wishes,

Tim Barnett

Tim Barnett
Department of Pathology
University of Tasmania
43 Collins Street
Hobart, Tas 7000
Ph:     +61-02-35-4825
Fax:    +61-02-35-4833
email:  t.barnett at

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