IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

poly(A) RNA isolation, question?

Cathy Sprankle Sprankle at ciit.org
Wed Dec 6 21:26:46 EST 1995

In article <Pine.SUN.3.91.951206135604.14797A-100000 at verdi>,
hroychow at NMSU.EDU (Hiranya Roychowdhury) wrote:

> On 6 Dec 1995, Shei-Pon Tzung wrote:
> > Hi all:
> > 
> > What is the best commercially available poly(A) RNA isolation kit?
> > I have tried Promega and Qiagen, which gave me a yield about 0.2 to 0.6 
> > %? I have been struggling with this for months, Please help.
> > Thanks.
> > 
> >        Shie-Pon Tzung
> Moving out of the 'kit-based' mindset would be a first step, I believe. 
> The good old oligo-dT cellulose (or sepharose, although I prefer the 
> former), in a 1-2 mL column made out of sterile 3 mL syringe and glass-wool 
> has always worked with excellent recovery.
I agree.  I've been very happy with bulk oligo(d)T cellulose from GIBCO/BRL, 
and Bio-Rad polypropylene 12 ml columns (can't remember the exact product
name) work very well for me.  I tried spin columns from various sources
several times and was never happy with them; the amount of handling
involved in using them seems to provide opportunities for contamination,
leaving you with poor quality RNA.

Good luck,

Catherine Sprankle
e-mail:  sprankle at ciit.org
Opinions expressed (such as they are!) are strictly mine and do
not reflect those of CIIT.

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net